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Measurement of cell viability, apoptosis and glutathione reductase activity HepG2 cells (purchased from Shanghai Institutes for Biological Sciences, Shanghai, China) were cultured in type B medium containing 12.76 nmol/L riboflavin before being transferred into the media with different riboflavin concentrations (0.76, 3.76, 12.76, 24.76, 48.76 nmol/L)